Evaluation of Marketed Polyherbal Antidiabetic Formulations using Biomarker Charantin

Abstract: Charantin is one of the phytoconstituents present in Momordica charantia Linn. M. charantia is known for its hypoglycemic activity from ancient times. In the present study an attempt has been made to develop a HPTLC method for quantitative estimation of charantin in small, big, fresh fruits and different marketed antidiabetic polyherbal formulations (PHF). Silica gel 60 F254 precoated plates (10 x 10 cm) were used with benzene: methanol (80:20) as solvent system. Sample was spotted on precoated TLC plates by using Linomat 5 spotter. Ascending mode was used for development of thin layer chromatography. TLC plates were developing up to 8 cms. The plates were sprayed with 10% sulphuric acid in alcohol and the reagent must be prepared freshly, heated at 1300 C for 2-3 min and brought to room temperature. Violet spot with Rf 0.32 was visible and scanned under 536 nm. This HPTLC method was found to be reproducible, accurate and precise and could detect charantin concentration at nanogram level. The developed HPTLC method would be an important tool in the way of acceptability of quality control method of polyherbal formulations. Data revealed that phfl contained highest quantity of the charantin. We found little less amount of charantin in phfd and phfj. It may be due to varied reasons like time of collections, age of the pant, processing conditions, incorrect identification of the plant, improper selection of the herb variety, addition of exhausted material and genetic variety of the plat material.

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