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THE PHARMA REVIEW (JULY - AUGUST 2010)

Spectrophotometric Analysis of Multi-component Formulations: An Overview

Rajkumar Dube, Sujata R. Pawar, Hardik R. Mody, Akshada Joshi, Vijaya Krishnan

Abstract
Pharmaceutical formulations with drug combinations in single dosage forms have gained increasing acceptance in the healthcare domain. This has posed a big challenge not only amongst pharmaceutical analysts to design robust and accurate methods with negligible overlapping effects on quantification but also amongst pharmaceutical administrators in particular to fight against counterfeit drugs. The present study is a compilation of the fundamentals of various Spectrophotometric methods used to carry out multi-component analysis. These include simultaneous equation method, area under the curve method, two wavelength method, absorbance ratio method, difference & derivative spectrophotometry and orthogonal polynomial method. The study also outlines various validation parameters which forms an integral part of the above mentioned methods.

 

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