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THE PHARMA REVIEW (JULY AUGUST 2014)

Ultra Performance Liquid Chromatography and Its Implementation - Current Scenario

Manu Grover

Introduction: To effectively leverage the power of smaller particles in narrow column formats while accelerating flow rate requires systems that operate at higher pressures and that are designed for both lower volumes and lower dispersion. The first design considerations involved the quality of tubing that could withstand UPLC operating conditions, so as not to dilute the magnitude gain in resolution brought about by UPLC column, hence extra-column volumes were minimized. 

 

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