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THE PHARMA REVIEW (JUNE 2008) |
RP-HPLC Estimation of
Eplerenone in Tablets
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K. P. Bhusari, P. B.
Khedekar, N. D. Amnerkar, S. M. Dhole, V. S. Banode |
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Abstract: A method
for the determination of Eplerenone in tablets has been
developed using reverse phase high performance liquid
chromatographic method. The elution was done using a
mobile phase consisting of methanol and water (75 : 25
v/v) on RP-C18 250 x4.6 mm analytical column with flow
rate of 1 ml/min with detection at 243 nm. The elution
time was 4.02 min. The method was found suitable for
quantitative estimation of eplerenone in tablets.
Introduction
Eplerenone (EPL) is chemically
9a,11a-Epoxy-7a-methylcarboxy-3-oxo-17a-pregn-4-en-21,17b
carbolactone, which is used as selective aldosterone
bloker, antihypertensive and diuretic.1,2 Eplerenone
binds to the mineralocorticoid receptor and blocks the
binding of aldosterone, a component of
renin-angiotensin-aldosterone system.3,4 It is listed in
Martindale.5 Literature survey revealed SPE LC-MS/MS
assay for eplerenone and its hydrolysed metabolite in
human urine.6 The present work describes the simple,
isocratic RP-HPLC method for the determination of EPL in
tablets. The developed method was validated using ICH
guidelines for validation.7
Materials and Method
The drug sample, Eplerenone, was obtained as gift sample
from MSN Lab. Pvt. Ltd., Medak. HPLC grade methanol and
water were supplied by Merck Co, Mumbai.
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