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THE PHARMA REVIEW (JUNE 2008)

UV-Spectrophotometry and First Order Derivative Methods for Estimation of Efavirenz in Bulk and Capsules

Charushila H. Bhirud, Atul A. Shirkhedkar, Ravindra A. Fursule and Sanjay J. Surana

Abstract: Two simple, rapid, accurate and economical 'UV Spectrophotometry' and 'First order derivative' methods have been developed for determination of efavirenz in bulk and capsules. In acetonitrile, the gmax of the drug was found to be 247 nm. The same spectrum was derivatised into first order derivative using UV probe software of instrument (Shimadzu- 2450), at Δλ = 2. The amplitude of the trough was recorded at 258 nm. Efavirenz follows linearity in the concentration range 4 - 24 g/ml in both these methods with correlation coefficient 0.9999 and 0.9998. The assay results were in good agreement with label claim. The methods were validated statistically and by recovery studies. The relative standard deviation were found to be less than 2% with excellent precision and accuracy.

Introduction
Efavirenz, (S) 6 chloro (cyclopropylethynyl)- 1,4–dihydro -4-(trifluromethyl)- 2H- 3,–1 benzoxazin–2 - one is non-nucleotide reverse transcriptase inhibitor and used in the treatment of immunodeficiency virus. In literature, few chromatographic3-5 methods are reported for estimation of efavirenz from human plasma.

The objective of the present work is to develop simple, rapid and economical 'UV-spectrophotometric' and 'first order derivative' methods for determination of efavirenz in bulk and finished products.

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