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THE PHARMA REVIEW (JUNE 2008) |
UV-Spectrophotometry and
First Order Derivative Methods for Estimation of
Efavirenz in Bulk and Capsules
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Charushila H. Bhirud, Atul
A. Shirkhedkar, Ravindra A. Fursule and Sanjay J. Surana |
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Abstract: Two simple,
rapid, accurate and economical 'UV Spectrophotometry'
and 'First order derivative' methods have been developed
for determination of efavirenz in bulk and capsules. In
acetonitrile, the gmax of the drug was found to be 247
nm. The same spectrum was derivatised into first order
derivative using UV probe software of instrument
(Shimadzu- 2450), at Δλ = 2. The amplitude of the trough
was recorded at 258 nm. Efavirenz follows linearity in
the concentration range 4 - 24 µg/ml in both these
methods with correlation coefficient 0.9999 and 0.9998.
The assay results were in good agreement with label
claim. The methods were validated statistically and by
recovery studies. The relative standard deviation were
found to be less than 2% with excellent precision and
accuracy.
Introduction
Efavirenz, (S) 6 chloro (cyclopropylethynyl)- 1,4–dihydro
-4-(trifluromethyl)- 2H- 3,–1 benzoxazin–2 - one is
non-nucleotide reverse transcriptase inhibitor and used
in the treatment of immunodeficiency virus. In
literature, few chromatographic3-5 methods are reported
for estimation of efavirenz from human plasma.
The objective of the present work is to develop simple,
rapid and economical 'UV-spectrophotometric' and 'first
order derivative' methods for determination of efavirenz
in bulk and finished products.
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