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Abstract
The aim of this study was to formulate and evaluate the
noble herbal moisturise gel containing Stevia extract.
The cosmetic gel formulation was designed by using
aqueous extract of Stevia rebaudiana leaves in varied
concentrations (2.5%, 5.0% and 10.0%) and evaluated
using physiological measurements. The gel was prepared
by using various polymer bases (HPMC, Sodium CMC and
Carbopol 934). Among them Carbopol 934 (Polymer grade)
has given better gel formation at particular (1.0%)
concentration. The gel was prepared by utilizing
Carbopol 934, Stevia extract, Propylene glycol 400 (5%),
Methyl paraben (0.5%), and required amount of distilled
water. Finally skin pH (6.8-7.0) was maintained by drop
wise addition of tri-ethanolamine solution. The
physicochemical parameters of formulations SF-I to
SF-III, i.e. pH, viscosity, spreadability, extrudability
were found to be in the range of 6.89 ± 0.20 – 6.98 ±
0.12, 7853- 24580 cps (at different RPM using spindle #
7, 250C), 27.32- 30.03 % and 89.21- 91.73% respectively.
Stability study have carried out as per ICH guideline
for 3 months at different temperatures and results
revealed formulation containing 2.5% Stevia extract
showed comparatively better stability than other
formulations and control sample. Further all the
formulations have studied for toxicity and skin
irritancy on animal model (Rabbit) before application to
the human volunteers. Results show there were no
toxicity and no skin irritation to the animals by
evaluated various parameters (PDI scores of eythema/edema,
behavioral observations, body weight determination,
hematological & biochemical parameters and
histopathological analysis)
when compared with control formulation.
Introduction
Herbals are the oldest form of primary healthcare which
had been used by all cultures throughout history. It was
an integral part of the development of modern
civilization. Oflate Stevia is a plant which was
originated from South America (Paraguay and Brazil),
belongs to the family Compositae (Bertoni, 1905). The
Asian markets consume over 85% of the global supply of
the fluffy white crystalline Stevia extracts. The
current extract market is 1.5 million kg, processed from
12 million kg of Stevia leaf and use for various
preparations. Stevioside is one of the important active
constituents which is abundantly available in the leaf
of Stevia (5-10% of dry weight basis). The focus of the
study is not only to substantiate Stevia’s effect as
calorie substitute and sweetener but also to justify the
multiple uses of stevioside in particular. Stevia acts
as flavor enhancer and in medicinal field it has
hypoglycemic, oral contraceptive, cardiovascular,
antimicrobial activity. It is also used to weight loss,
digestive and skin problems (Mourey, 1992; Ghosh et al.,
2008 and Kuntal et al, 2009). There are scanty reports
on Stevia as herbal gel preparation. A report on gel
formulation containing 5, 10, 15 % crude Stevia extract
and 5 and 10% of pure stevioside were prepared and their
physical stability were established (Manosroi et al.,
1999). Literature survey revealed that stevioside
(active constituent of Stevia plant) is a useful potent
drug (Shawn, 1949). Looking at that, in a part of Ph. D
study, successful attempts have been made for the first
time in India to establish only the role of Stevia as
skin tonner. Hence various formulations were prepared
from the cultivated Stevia in South India (Ripponpet,
Shimoga Dist, Karnataka) and tested for pH, stability,
viscosity, tonicity, spreadibility and other parameters.
Further preclinical study was carried out and
investigated toxicity and irritancy for safely study of
gel formulations that will cover all the aspects of
improving healthy skin.
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