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THE PHARMA REVIEW (MAY - JUNE 2011)

Spectrophotometric Estimation of Naratriptan: Influence of Solvents

Murthy. T. E. G. K, Veditha. K

Abstract: A simple, sensitive, selective, accurate, precise, economical, robust and rapid UV specctrophotometric method is developed and validated for quantification of naratriptan. The influence of three different solvents on estimation of naratriptan from bulk and pharmaceutical dosage forms was studied. The solutions of naratriptan were prepared with the water and the solvent blends containing water: acetonitrile (1:1) and water: methanol (1:1). The solutions were scanned with in the ranges of 200 nm to 400nm. Naratriptan exhibited λmax at 283.5 nm in case of water, however the λmax was slightly shifted to 285 nm in case of acetonitrile: water and 284.5 in methanol: water. The method obeyed Beer-Lamberts law with in the range of 10 to 50 g/ml concentration. The analytical parameters such as linearity, A (1%, 1 cm), LOD, LOQ, inter day precision, intraday precision, molar absorption coefficient, and sandells sensitivity were calculated. The results of analysis were validated by recovery studies. The recovery was more than 97%. The validation parameters were treated statistically with ANOVA and there were significant differences noticed. This study clearly indicated that the solvent is having an influence in determination of naratriptan from bulk drug as well as from the formulations. The described method has the advantage of being rapid and easy. Hence it can be applied for routine quality control analysis of naratriptan from pharmaceutical preparation.
Introduction
Naratriptan is a potent and selective agonist for vascular 5-HT1 receptors. It acts on 5-HT1 receptors on peripheral and central terminals of the trigeminal nerve to inhibit trigeminal nerve activity. This may lead to anti migraine activity in humans. The chemical name of naratriptan is N-methyl-3-(1-methyl-4-piperidyl)indole-5-ethanesulfonamide.molecular formula of naratriptan is C17H25N3O2S with a molecular weight of 371.9. Naratriptan possesses no chiral centers.1-2
Structure of Naratriptan
There are few GC3 HPLC,4 voltammetry method5 and visible spectrophotometric methods6 were reported for naratriptan. UV methods were not reported for the estimation of naratriptan. The position as well as the intensity of absorption maximum gets shifted for a particular chromophore by changing the polarity of the solvent. By increasing the polarity of the solvent, compounds such as dienes and conjugated hydrocarbons do not experience any appreciable shift. In general, the absorption maximum for the non polar compounds is usually shifted with the change in polarity of the solvents. α, β unsaturated carbonyl compounds show two different shifts.7 The choice of solvents for use in a spectrophotometric analysis is limited primarily by the solubility of the analyte. However the absorbance of the solvent at the analytical wave length is an important limiting factor and should not absorb radiations above 210 nm.8 The solvent selection can be narrowed further by considering the extraneous materials such as excipients, degradation products, or contaminants that might be present in the sample. So studies were under taken to compare the efficiency of three different solvents on estimation of naratriptan and the results are reported here.

 

 

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