Abstract: A simple rapid, accurate and economical ‘First order UV-derivative spectrophotometric’ method has been developed for estimation of Nebivolol from pharmaceutical formulations. In methanol-water (3:7) solvent, Nebivolol showed maximum absorbance at 281 nm. In first order derivative spectrum amplitude of the trough was recorded at 291nm. Linearity was observed at 291 nm in the concentration range of 10 - 70 µg/mL (r2=0.9997). The amount of drug estimated from the tablet formulation was found to be in the good agreement with the label claim. The method has been validated statistically and by recovery studies.
Introduction: Nebivolol hydrochloride (NEB), 1-(6-flurochroman-2yl) – {[2-(6-flurochroman-2-yl)-2 hydroxyethyl] amino} ethanol, Hcl is antihypertensive. Literature survey revealed few analytical methods which include liquid chromatography with tandem mass spectrometry, liquid chromatography coupled with electro spray ionization tandem mass sectroscoy for estimation of Nebivolol in biological fluids. RP-HPLC method has also been reported for estimation of Nebivolol from tablet formulations. Nebivolol is not official in IP, BP and USP.
The aim of the present work is to develop First order spectrophotometric method and validate it for linearity, accuracy, precision, ruggedness and robustness. |
